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  • Title: On-line stacking and sweeping capillary electrophoresis for detecting heroin metabolites in human urine.
    Author: Jong YJ, Ho YH, Ko WK, Wu SM.
    Journal: J Chromatogr A; 2009 Oct 30; 1216(44):7570-5. PubMed ID: 19439308.
    Abstract:
    Heroin metabolites including morphine, codeine, and 6-acetylmorphine were determined by cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography (CSEI-sweep-MEKC). Liquid-liquid extraction was used for urine pretreatment. An uncoated fused silica capillary (Ld=30 cm, 50 microm ID) was filled with phosphate buffer (50 mM, pH 2.5) containing 30% methanol, then high conductivity buffer (100 mM phosphate, 41.3 kPa for 18 s) was followed. Samples were injected electrokinetically (20 kV, 300 s). The sweeping and separation were performed at -25 kV using phosphate buffer (20 mM, pH 2.5) and 80 mM sodium dodecyl sulfate. The baseline separation was done within 10 min. During method validation, the calibration curves were linear over a range of 50-500 ng/mL (r > or = 0.994). The RSD and RE values in intra-day and inter-day assays were all below 20%, which showed good precision and accuracy. Their detection limits were 10 ng/mL (S/N=3). The optimized method was applied to determine real urine samples from addicts. These samples were confirmed by liquid chromatography/mass spectrometry.
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