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  • Title: [Determination of oleuropein in stem of Syringa oblata from different districts by HPLC].
    Author: Wei X, Zhang S, Zhai W, Zhang S, Wang Z.
    Journal: Zhongguo Zhong Yao Za Zhi; 2009 Feb; 34(3):304-6. PubMed ID: 19445155.
    Abstract:
    OBJECTIVE: To develop an HPLC method for the determination of oleuropein in Syringa oblata. METHOD: An Aigilent ZORBAX SB- C18 column (4.6 mm x 250 mm, 5 microm) was used. The mobile phase was acetonitrile-water (21 : 79). The flow rate was 1.0 mL x min(-1). The detection wavelenghth was set at 232 nm and the column temperature was 30 degrees C. RESULT: The linear range of oleuropein were from 0.011 62 g x L(-1) to 1.162 g x L(-1). The average recovery was 98.7% with RSD 2.5% (n=9). CONCLUSION: The method is reliable, accurate and specific. It can be used for quality control of the stem of Syringa oblata.
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