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  • Title: [Effect of clpE gene deletion on virulence of Streptococcus pneumoniae].
    Author: Zhang Q, Yin N, Xu W, Wang H, Pang D, Yang X, Yin Y, Zhang X.
    Journal: Wei Sheng Wu Xue Bao; 2009 Feb; 49(2):233-8. PubMed ID: 19445180.
    Abstract:
    OBJECTIVE: To study the effect of clpE gene deletion on the virulence of Streptococcus pneumoniae. METHODS: The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition,we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase. RESULTS: The clpE gene was replaced completely by erm cassette. Mice virulence experiments showed that the median lethal time of the wide-type was 54 h, whereas that of clpE mutant was 21d (P < 0.01). Cell culture infection experiments indicated that adherence and invasion of clpE mutant were strongly reduced (P < 0.05). The expression of virulent factors in clpE mutant was lower than that of the wild-type (P < 0.05). CONCLUSION: ClpE is involved in virulence by modulating the expressions of virulence factors.
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