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  • Title: The interaction of Clostridium perfringens and its toxins in the production of necrotic enteritis of chickens.
    Author: Al-Sheikhly F, Truscott RB.
    Journal: Avian Dis; 1977; 21(2):256-63. PubMed ID: 194572.
    Abstract:
    The intraduodenal administration of large numbers of Clostridium perfringens cells harvested from broth cultures and resuspended in PBS or fresh sterile thioglycollate broth produced a very mild form of necrotic enteritis. Administering an appropriate number of cells in culture supernatant, however, produced typical field-type disease. Alpha toxin was shown to be the significant toxin recoverable from broth-culture supernatant fluids. Requirements to produce the disease are minor intestinal damage and sufficient numbers of toxigenic C. perfringens in the intestine.
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