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  • Title: Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform.
    Author: Kim YG, Jeong HJ, Jang KS, Yang YH, Song YS, Chung J, Kim BG.
    Journal: Anal Biochem; 2009 Aug 15; 391(2):151-3. PubMed ID: 19457428.
    Abstract:
    We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures. The released N-glycans from polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan preparation technology is applied to ovarian cancer diagnosis using 5 microl of patient serum.
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