These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Detection of antimalarial activity for new compounds by isotopic microtest].
    Author: Chen ZG, Moreno A, Benito A, Moreno M, Berzosa PJ, de Lucio A, Moyano E.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 2009 Feb 28; 27(1):83-6. PubMed ID: 19459510.
    Abstract:
    Two clone lines (Dd2 and 3D7) of Plasmodium falciparum were cultivated continuously in human erythrocytes at 37 degrees C in RPMI 1640 medium with human serum and subjected to 6% sorbitol treatment 2 times in order to obtain highly synchronized cultures. The second generation parasites after the treatment were diluted with human RBC to be a suspension of P. falciparum-human RBC at 2.5% hematocrit and 0.5% parasitemia, and 2 microCi/ml of 8-3H-hypoxanthine was added. Isotopic microtest was employed to detect the antimalarial activity for 20 new compounds. Results revealed that the 20 compounds showed no anti-malarial activity, while the control drugs, chloroquine and quinine, exhibited high efficacy, indicating that the isotopic microtest is a stable and reproducible assay for screening new antimalarials.
    [Abstract] [Full Text] [Related] [New Search]