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  • Title: Genome-wide gene expression profiling in gamma-irradiated green alga, Pseudokirchneriella subcapitata, by HiCEP.
    Author: Fuma S, Nakamori T, Ishii N, Kubota Y, Yoshida S, Fujimori A.
    Journal: Bull Environ Contam Toxicol; 2009 Sep; 83(3):301-6. PubMed ID: 19462152.
    Abstract:
    Transcriptome was analyzed in gamma-irradiated green alga Pseudokirchneriella subcapitata by high coverage gene expression profiling (HiCEP). Approximately 7,800 expressed genes were detected. Expression levels of 623-707 genes were affected at 100-300 Gy. Nucleotide sequences of 41 up-regulated genes were determined. The quantitative reverse transcription polymerase chain reaction validated the up-regulation. Two genes had homology to genes related to ionizing radiation. These results indicate usefulness of HiCEP for screening of stress-responsive genes in species that are ecotoxicologically important but for which genomic sequence information is lacking.
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