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  • Title: Differential effects of a transgene to confer low phytic acid in caryopses located at different positions in rice panicles.
    Author: Kuwano M, Takaiwa F, Yoshida KT.
    Journal: Plant Cell Physiol; 2009 Jul; 50(7):1387-92. PubMed ID: 19465440.
    Abstract:
    In previous studies, we attempted to reduce phytic acid in rice seeds by silencing the 1d-myo-inositol 3-phosphate synthase gene, RINO1, using an antisense sequence under the control of the rice glutelin GluB-1 promoter. The stable transgenic line showed a weak low phytic acid phenotype. In this study, we show that the position of the caryopsis in the panicle might affect the level of gene silencing through a difference in temporal and spatial expression patterns between RINO1 and GluB-1 promoters, resulting in a large variation in Pi levels and a small increase in Pi in the transgenic seeds.
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