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  • Title: The chitin-binding capability of Cy-AMP1 from cycad is essential to antifungal activity.
    Author: Yokoyama S, Iida Y, Kawasaki Y, Minami Y, Watanabe K, Yagi F.
    Journal: J Pept Sci; 2009 Jul; 15(7):492-7. PubMed ID: 19466694.
    Abstract:
    Antimicrobial peptides are important components of the host innate immune responses by exerting broad-spectrum microbicidal activity against pathogenic microbes. Cy-AMP1 found in the cycad (Cycas revoluta) seeds has chitin-binding ability, and the chitin-binding domain was conserved in knottin-type and hevein-type antimicrobial peptides. The recombinant Cy-AMP1 was expressed in Escherichia coli and purified to study the role of chitin-binding domain. The mutants of Cy-AMP1 lost chitin-binding ability completely, and its antifungal activity was markedly decreased in comparison with native Cy-AMP1. However, the antimicrobial activities of the mutant peptides are nearly identical to that of native one. It was suggested that the chitin-binding domain plays an essential role in antifungal, but not antimicrobial, activity of Cy-AMP1.
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