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  • Title: Single-step purification of native miraculin using immobilized metal-affinity chromatography.
    Author: Duhita N, Hiwasa-Tanase K, Yoshida S, Ezura H.
    Journal: J Agric Food Chem; 2009 Jun 24; 57(12):5148-51. PubMed ID: 19469504.
    Abstract:
    Miraculin is a taste-modifying protein that can be isolated from miracle fruit ( Richadella dulcifica ), a shrub native to West Africa. It is able to turn a sour taste into a sweet taste. The commercial exploitation of this sweetness-modifying protein is underway, and a fast and efficient purification method to extract the protein is needed. We succeeded in purifying miraculin from miracle fruit in a single-step purification using immobilized metal-affinity chromatography (IMAC). The purified miraculin exhibited high purity (>95%) in reverse-phase high-performance liquid chromatography. We also demonstrated the necessity of its structure for binding to the nickel-IMAC column.
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