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  • Title: Phospholipase C from Clostridium perfringens stimulates formation and release of platelet-activating factor (PAF-acether) in cultured intestinal epithelial cells (INT 407).
    Author: Gustafson C, Kald B, Sjödahl R, Tagesson C.
    Journal: Scand J Gastroenterol; 1991 Oct; 26(10):1000-6. PubMed ID: 1947765.
    Abstract:
    This study demonstrates the ability of phospholipase C from Clostridium perfringens to stimulate the generation of platelet-activating factor (PAF-acether) in cultured intestinal epithelial cells (INT 407). Cells were exposed to phospholipase C for up to 60 min, and the content of PAF-acether within the cells and in the extracellular medium was determined. Phospholipase C caused a time-dependent formation of PAF-acether within the cells and also release of PAF-acether to the medium. In contrast, phospholipase C did not affect the cellular acetylhydrolase activity or the ability of the cells to metabolize extracellularly added 14C-PAF-acether. These findings suggest the possibility that intestinal epithelial cells, when stimulated with a naturally occurring intestinal bacterial toxin, generate and release PAF-acether. The possibility that this might contribute to the pathophysiology of inflammatory bowel disease is discussed.
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