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  • Title: In vitro and in vivo fertility of ram semen cryopreserved in different extenders.
    Author: Valente SS, Pereira RM, Baptista MC, Marques CC, Vasques MI, Pereira MV, Horta AE, Barbas JP.
    Journal: Anim Reprod Sci; 2010 Jan; 117(1-2):74-7. PubMed ID: 19482446.
    Abstract:
    Seminal traits of frozen-thawed (FT) ram semen and in vitro and field fertility in native Portuguese breeds were evaluated in 4 experiments. In exp. 1 and 2 the cryopreservation capacity of 2 extenders, E1 (15% egg yolk-EY) and E2 (4.5% EY and trehalose) was compared through morphological evaluation and in vitro fertilizability of FT ram semen. Exp. 3 aimed to determine the usefulness of in vitro homologous/heterologous fertilization tests as tools for predicting ram fertility. Exp. 4 was conducted to verify if the identified differences between the 2 extenders could be confirmed by field fertility. E1 showed a better cryoprotective action expressed by higher in vitro and field fertility results. In conclusion, EY is difficult to be replaced in ram semen extenders. Heterologous fertilization seems to be a useful tool for predicting fertility of FT ram semen.
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