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  • Title: Axonal regeneration induced by repetitive electrical stimulation of crushed optic nerve in adult rats.
    Author: Tagami Y, Kurimoto T, Miyoshi T, Morimoto T, Sawai H, Mimura O.
    Journal: Jpn J Ophthalmol; 2009 May; 53(3):257-66. PubMed ID: 19484445.
    Abstract:
    PURPOSE: To investigate whether electrical stimulation promoted axonal regeneration of retinal ganglion cells (RGCs) after optic nerve (ON) crush in adult rats. METHODS: Transcorneal electrical stimulation (TES), which stimulates the retina with current from a corneal contact lens electrode, was used to stimulate the eye. TES was applied for 1 h immediately after ON crush. Axonal regeneration was determined by anterograde labeling of RGC axons. To examine whether the axonal regeneration was mediated by insulin-like growth factor 1 (IGF-1) receptors, an IGF-1 receptor antagonist, JB3, was injected intraperitoneally before each TES application. Immunostaining for IGF-1 was performed to examine the effects of TES. To test the survival-promoting effects of TES applied daily, the mean density of retrogradely labeled RGCs was determined on day 12 after ON crush. RESULTS: Compared with sham stimulation, the mean number of regenerating axons significantly increased at 250 microm distal from the lesion and increased IGF-1 immunoreactivity was observed in retinas treated daily with TES. Preinjection of an IGF-1 receptor antagonist significantly blocked axonal regeneration by TES applied daily. TES applied daily also markedly enhanced the survival of RGCs 12 days after ON crush. CONCLUSION: TES applied daily promotes both axonal regeneration and survival of RGCs after ON crush.
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