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  • Title: 3-(10'-Phenothiazinyl)propane-1-sulfonate is a potent enhancer of soybean peroxidase-induced chemiluminescence.
    Author: Vdovenko MM, Ciana LD, Sakharov IY.
    Journal: Anal Biochem; 2009 Sep 01; 392(1):54-8. PubMed ID: 19500555.
    Abstract:
    3-(10'-Phenothiazinyl)propane-1-sulfonate (SPTZ) was shown to be a potent enhancer of soybean peroxidase (SbP)-induced chemiluminescence. To the best of our knowledge, SPTZ is the first enhancer of SbP to be discovered. Optimal conditions for SbP-catalyzed oxidation of luminol in the presence of SPTZ were determined. The SbP-SPTZ system showed better sensitivity and a lower detection limit (LDL) with respect to the horseradish peroxidase-4-iodophenol system traditionally used in chemiluminescent enzyme-linked immunosorbent assay (ELISA). The addition of 4-morpholinopyridine (MORP) to the SbP-SPTZ system improved its analytical parameters by decreasing the LDL of SbP to 0.03pM. These results open up very promising perspectives for using the SbP-SPTZ-MORP system in ultrasensitive immunoassay.
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