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  • Title: Increased inhibitory ability of conjugated RNA aptamers against the HCV IRES.
    Author: Kikuchi K, Umehara T, Nishikawa F, Fukuda K, Hasegawa T, Nishikawa S.
    Journal: Biochem Biophys Res Commun; 2009 Aug 14; 386(1):118-23. PubMed ID: 19501043.
    Abstract:
    Hepatitis C virus (HCV) translation begins within the internal ribosome entry site (IRES). We have previously isolated two RNA aptamers, 2-02 and 3-07, which specifically bind to domain II and domain III-IV of the HCV IRES, respectively, and inhibit IRES-dependent translation. To improve the function of these aptamers, we constructed two conjugated molecules of 2-02 and 3-07. These bound to the target RNA more efficiently than the two parental aptamers. Furthermore, they inhibited IRES-dependent translation about 10 times as efficiently as the 3-07 aptamer. This result indicates that combining aptamers for different target recognition sites potentiates the inhibition activity by enhancing the domain-binding efficiency.
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