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  • Title: [Study on the inducement and influential factors of callus with different organs of Glycyrrhiza uralensis].
    Author: Fan XF, Yang YL, Guo XQ, Liu XL.
    Journal: Zhong Yao Cai; 2009 Feb; 32(2):173-6. PubMed ID: 19504955.
    Abstract:
    OBJECTIVE: To investigate the influential factors and establish culture method for G. uralensis callus. METHODS: To study the possible effective factors of culture condition by comparing with different explants, light, plant hormones and its ratio. RESULTS: The hypocotyl was the best among different explants, its inducing ratios was 94%, and the callus occurred earliest. When the calluses were inoculated on MS + 6 - BA (1.0-2.0) mg/L + NAA (0.5-1.0) mg/L, adventitious buds formed. 2,4-D could induce non-embryogenic callus, the compounding proportions of 6 - BA and NAA could induce embryogenic callus. Light influenced induction and growth of callus. CONCLUSION: Different explants, components of hormone and light are the influencial factors of callus induction and growth of G. uralensis.
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