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  • Title: Stabilizing the urinary activity of fructose-1,6-bisphosphatase with EDTA and mercaptoethanol.
    Author: Kepka A, Szajda SD, Waszkiewicz N, Zalewska A, Płudowski P, Zwierz-Gugała D, Zalewska-Szajda B, Borzym-Kluczyk M, Kryśkiewicz E, Zwierz K.
    Journal: Clin Biochem; 2009 Sep; 42(13-14):1487-9. PubMed ID: 19505453.
    Abstract:
    OBJECTIVES: The study aim was to establish conditions for stabilization the activity of fructose-1,6-bisphosphatase (FBP-1) in stored urine. DESIGN AND METHODS: The FBP-1 was determined by the method Kepka et. al in a collected fraction of purified urine. RESULTS: EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine. CONCLUSION: At optimal conditions urine may be stored up to 7 days at a temperature of 4 degrees C.
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