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  • Title: Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by beta-oxidation-impaired mutant of Pseudomonas putida KT2442.
    Author: Ma L, Zhang H, Liu Q, Chen J, Zhang J, Chen GQ.
    Journal: Bioresour Technol; 2009 Oct; 100(20):4891-4. PubMed ID: 19505819.
    Abstract:
    Pseudomonas putida KT2442 produces medium-chain-length (MCL) polyhydroxyalkanoates (PHA) from fatty acids. When gene encoding 3-hydroxyacyl-CoA dehydrogenase which catalyzes long-chain-3-hydroxyacyl-CoA to 3-ketoacyl-CoA, was partially or completely deleted in P. putida KTOY08, the PHA accumulated was shown to contain only two different monomer structures dominated by a monomer of the same chain length as that of the fatty acids fed and another monomer two carbon atoms shorter. Among the PHA copolymers, P(44% 3HD-co-3HDD) containing 44% 3HD and 56% 3HDD was demonstrated to have a melting temperature T(m), an apparent heat of fusion DeltaH(m) and a Young's modulus E of 75 degrees C, 51 J g(-1) and 2.0 MPa, respectively, the highest among all the MCL PHA studied.
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