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  • Title: Determination of carnitine, its short chain acyl esters and metabolic precursors trimethyllysine and gamma-butyrobetaine by quasi-solid phase extraction and MS/MS detection.
    Author: Hirche F, Fischer M, Keller J, Eder K.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2009 Jul 15; 877(22):2158-62. PubMed ID: 19523885.
    Abstract:
    For the investigation of the metabolism and biosynthesis of carnitine, sensitive determination of carnitine and its metabolic precursors, trimethyllysine and gamma-butyrobetaine, is required. We present here a new simplified method for the analysis of carnitine, its acetyl- and propyl esters, as well as trimethyllysine and gamma-butyrobetaine without need for derivatization reactions by means of normal-phase LC and electrospray ionization tandem mass spectrometry. The limits of quantification were between 5 nM for acetyl carnitine and 70 nM for carnitine. Relative standard deviations in a fivefold determination of standard solutions were between <2% for carnitine and <10% for trimethyllysine. Quantifying the formation of deuterated carnitine from deuterated gamma-butyrobetaine, this method is also suitable for the determination of the activity of gamma-butyrobetaine dioxygenase in tissues.
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