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  • Title: On-line concentration of neutral analytes by complexation and acetonitrile sweeping in nonionic microemulsion electrokinetic chromatography with direct ultraviolet detection.
    Author: Cao J, Yi L, Li P, Chang YX.
    Journal: J Chromatogr A; 2009 Jul 17; 1216(29):5608-13. PubMed ID: 19524933.
    Abstract:
    To separate and detect neutral solutes in nonionic microemulsion electrokinetic chromatography (MEEKC), a novel method was developed, combining complex formation and acetonitrile (ACN) sweeping. In this report, dynamic borate complexation and on-line sweeping occurred simultaneously during a run. The operating parameters which affected the performance of analyte sweeping in nonionic MEEKC were examined in terms of borate complexation, ACN content, Brij-35 concentration and sample plug length. In addition, the validation of the method included tests of the limit of detection, reproducibility and sensitivity enhancement. 60-110-Fold of magnitude improvement in detection sensitivity for model compounds (ginsenoside Rf, ginsenoside Rb2, ginsenoside Re) using Brij-35 microemulsion was demonstrated. Furthermore, the method was applied to the determination of glucosides in the plant extract.
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