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  • Title: Identification of F strain Mycoplasma gallisepticum isolates by detection of an immunoreactive protein.
    Author: Thomas CB, Sharp P, Fritz BA, Yuill TM.
    Journal: Avian Dis; 1991; 35(3):601-5. PubMed ID: 1953583.
    Abstract:
    Commercial laying hens were examined microbiologically at necropsy 31 or 42 weeks after aerosol vaccination with the F strain of Mycoplasma gallisepticum (MG). Mycoplasma isolates were studied in Western blots probed with polyclonal antiserum raised in rabbits to F strain immunogen. The persistence of the vaccine strain was demonstrated by detection of a 75-kilodalton immunoreactive protein, which was present in all MG isolates and thought to be a unique marker of the F strain. Use of PCA-F to probe Western blots allowed simultaneous identification of non-MG isolates, non-F strains of MG, and the F strain of MG.
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