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  • Title: Application of a novel chemiluminescence-based DNA detection method to single-vector and multiplex DNA sequencing.
    Author: Creasey A, D'Angio L, Dunne TS, Kissinger C, O'Keeffe T, Perry-O'Keefe H, Moran LS, Roskey M, Schildkraut I, Sears LE.
    Journal: Biotechniques; 1991 Jul; 11(1):102-4, 106, 108-9. PubMed ID: 1954008.
    Abstract:
    A chemiluminescent DNA detection method is described and its application shown for both single-vector and multiplex DNA sequencing using the standard dideoxy chain-termination process. This recently developed detection method, which utilizes the light emitted by an enzyme-catalyzed dioxetane reaction, is highly sensitive and affords significant advantages in safety and speed over the traditional radioactive labeling method. When adapted to a multiplex strategy, this chemiluminescent detection method constitutes a safe, simple and rapid method for increasing the throughput of DNA sequencing procedures.
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