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  • Title: Determination of phenylalanine in blood by high-performance anion-exchange chromatography-pulsed amperometric detection to diagnose phenylketonuria.
    Author: Jeong JS, Sim HJ, Lee YM, Yoon HR, Lee DH, Hong SP.
    Journal: J Chromatogr A; 2009 Jul 24; 1216(30):5709-14. PubMed ID: 19540504.
    Abstract:
    We have developed a high-performance anion-exchange chromatography with pulsed amperometric detection method for the detection of phenylalanine (Phe) and diagnosis of phenylketonuria (PKU). Sample pretreatment steps were simplified without derivatization. The analyte was separated within 5 min. The detection limit (S/N=3) for Phe was 50 pg. Linear dynamic range was 1.23-14.43 mg/dL (r(2)=0.9999) for a dried blood spot. The mean recoveries of Phe for intra- and inter-day assays were found to be 96.87-104.16%. This method clearly differentiated PKU-positive groups from normal groups, and proved to be a practical procedure for rapid screening and follow-up monitoring of PKU.
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