These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Plasmid-based system for high-level gene expression and antisense gene knockdown in Bartonella henselae.
    Author: Gillaspie D, Perkins I, Larsen K, McCord A, Pangonis S, Sweger D, Seleem MN, Sriranganathan N, Anderson BE.
    Journal: Appl Environ Microbiol; 2009 Aug; 75(16):5434-6. PubMed ID: 19542333.
    Abstract:
    Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a beta-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of B. henselae, this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%.
    [Abstract] [Full Text] [Related] [New Search]