These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production.
    Author: Munro SA, Zinder SH, Walker LP.
    Journal: Biotechnol Prog; 2009; 25(4):1035-42. PubMed ID: 19551880.
    Abstract:
    The hyperthermophilic bacterium, Thermotoga neapolitana, has potential for use in biological hydrogen (H(2)) production. The objectives of this study were to (1) determine the fermentation stoichiometry of Thermotoga neapolitana and examine H(2) production at various growth temperatures, (2) investigate the effect of oxygen (O(2)) on H(2) production, and (3) determine the cause of glucose consumption inhibition. Batch fermentation experiments were conducted at temperatures of 60, 65, 70, 77, and 85 degrees C to determine product yield coefficients and volumetric productivity rates. Yield coefficients did not show significant changes with respect to growth temperature and the rate of H(2) production reached maximum levels in both the 77 degrees C and 85 degrees C experiments. The fermentation stoichiometry for T. neapolitana at 85 degrees C was 3.8 mol H(2), 2 mol CO(2), 1.8 mol acetate, and 0.1 mol lactate produced per mol of glucose consumed. Under microaerobic conditions H(2) production did not increase when compared to anaerobic conditions, which supports other evidence in the literature that T. neapolitana does not produce H(2) through microaerobic metabolism. Glucose consumption was inhibited by a decrease in pH. When pH was adjusted with buffer addition cultures completely consumed available glucose.
    [Abstract] [Full Text] [Related] [New Search]