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  • Title: Characterization of start-up performance and archaeal community shifts during anaerobic self-degradation of waste-activated sludge.
    Author: Kobayashi T, Yasuda D, Li YY, Kubota K, Harada H, Yu HQ.
    Journal: Bioresour Technol; 2009 Nov; 100(21):4981-8. PubMed ID: 19556123.
    Abstract:
    Successful start-up strategy for anaerobic digestion of waste-activated sludge using internal inoculum and relationship between the shift of methanogenic community and the digester performance during start-up was investigated. Combination of TS control of inoculum and batch operation during early days enabled the successful start-up operation without serious volatile fatty acid accumulation, followed by the stable continuous operation. However, the propionate degradation was rate-limiting step during the batch operation. The results of real-time quantitative polymerase chain reaction analysis suggested that there was a correlation between the population of the genus Methanosarcina and the methane production rate coupled with acetate consumption during batch operation, and the results of terminal-restriction fragment length polymorphism (T-RFLP) revealed that the increasing intensity of T-RF peaks of hydrogenotrophic methanogens was associated with a decrease in the level of C3-acids.
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