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  • Title: Antibacterial activity of recombinant hCAP18/LL37 protein secreted from Pichia pastoris.
    Author: Kim SJ, Quan R, Lee SJ, Lee HK, Choi JK.
    Journal: J Microbiol; 2009 Jun; 47(3):358-62. PubMed ID: 19557354.
    Abstract:
    Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZaA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.
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