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  • Title: Production of yellow fever virus in microcarrier-based Vero cell cultures.
    Author: Souza MC, Freire MS, Schulze EA, Gaspar LP, Castilho LR.
    Journal: Vaccine; 2009 Oct 30; 27(46):6420-3. PubMed ID: 19559120.
    Abstract:
    In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor to evaluate the use of a commercial serum-free medium (VP-SFM) and to investigate the effects of multiplicity of infection (MOI) and time of infection (TOI) on virus production. Virus titers as high as 8.4 x 10(8)pfu/mL were obtained upon infection with MOI of 0.02 and TOI of 3 days, using the serum-free medium in the sparged bioreactor.
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