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  • Title: ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification.
    Author: Stephan AB, Shum EY, Hirsh S, Cygnar KD, Reisert J, Zhao H.
    Journal: Proc Natl Acad Sci U S A; 2009 Jul 14; 106(28):11776-81. PubMed ID: 19561302.
    Abstract:
    For vertebrate olfactory signal transduction, a calcium-activated chloride conductance serves as a major amplification step. However, the molecular identity of the olfactory calcium-activated chloride channel (CaCC) is unknown. Here we report a proteomic screen for cilial membrane proteins of mouse olfactory sensory neurons (OSNs) that identified all the known olfactory transduction components as well as Anoctamin 2 (ANO2). Ano2 transcripts were expressed specifically in OSNs in the olfactory epithelium, and ANO2::EGFP fusion protein localized to the OSN cilia when expressed in vivo using an adenoviral vector. Patch-clamp analysis revealed that ANO2, when expressed in HEK-293 cells, forms a CaCC and exhibits channel properties closely resembling the native olfactory CaCC. Considering these findings together, we propose that ANO2 constitutes the olfactory calcium-activated chloride channel.
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