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  • Title: Functional and structural characterization of a flavonoid glucoside 1,6-glucosyltransferase from Catharanthus roseus.
    Author: Masada S, Terasaka K, Oguchi Y, Okazaki S, Mizushima T, Mizukami H.
    Journal: Plant Cell Physiol; 2009 Aug; 50(8):1401-15. PubMed ID: 19561332.
    Abstract:
    Sugar-sugar glycosyltransferases play an important role in structural diversity of small molecule glycosides in higher plants. We isolated a cDNA clone encoding a sugar-sugar glucosyltransferase (CaUGT3) catalyzing 1,6-glucosylation of flavonol and flavone glucosides for the first time from Catharanthus roseus. CaUGT3 exhibited a unique glucosyl chain elongation activity forming not only gentiobioside but also gentiotrioside and gentiotetroside in a sequential manner. We investigated the functional properties of CaUGT3 using homology modeling and site-directed mutagenesis, and identified amino acids positioned in the acceptor-binding pocket as crucial for providing enough space to accommodate flavonoid glucosides instead of flavonoid aglycones. These results provide basic information for understanding and engineering the catalytic functions of sugar-sugar glycosyltransferases involved in biosynthesis of plant glycosides.
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