These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Chiral ligand-exchange CE assays for separation of amino acid enantiomers and determination of enzyme kinetic constant.
    Author: Qi L, Qiao J, Yang G, Chen Y.
    Journal: Electrophoresis; 2009 Jul; 30(13):2266-72. PubMed ID: 19569124.
    Abstract:
    This paper deals with studies on the use of Zn(II)-L-ornithine complex as a chiral selecting system for the enantioseparation and UV detection of amino acids (AAs) by using the principle of ligand-exchange CE. Successful enantioseparation of three pairs of label-free aromatic AAs and four pairs of labeled AA enantiomers have been achieved with a buffer of 100.0 mM boric acid, 5.0 mM ammonium acetate, 3.0 mM ZnSO4 and 6.0 mM L-Orn at pH 8.2. This new method was shown to be applicable to the quantitative analysis of D- and L-aromatic AAs, with a linear range between 12.5 and 800.0 microg/mL, and a correlation coefficient above 0.99. Thus this assay, which is facile and relatively rapid, allows us to measure the enzyme catalytic activity in the incubation of D,L-AAs with D-AA oxidase. Using this new method, we can determine the enzyme kinetic constant, lending insight into potential enzyme mechanism.
    [Abstract] [Full Text] [Related] [New Search]