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  • Title: Development and validation of LC-MS/MS method for the determination of cyproheptadine in several pharmaceutical syrup formulations.
    Author: Feás X, Ye L, Hosseini SV, Fente CA, Cepeda A.
    Journal: J Pharm Biomed Anal; 2009 Dec 05; 50(5):1044-9. PubMed ID: 19574011.
    Abstract:
    A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for CP and 282.1/167.2 and 282.1/116.3 for DPP. The retention time of the drug was 7.29 min. The analytical method was successfully validated for linearity (1-100 ng/ml), intra-day precision, inter-day precision, and accuracy. The limit of detection (LOD) and limit of quantification (LOQ) were 0.86 and 0.98 ng/ml, respectively. The proposed method was applied to analyse the cyproheptadine content from seven different syrup formulations.
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