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  • Title: [Berberine-electrochemical detector for the determination of berberine-type alkaloids in various Chinese patent medicines by flow injection analysis].
    Author: Liu WZ, Peng WB, Yang CH.
    Journal: Yao Xue Xue Bao; 1991; 26(4):315-9. PubMed ID: 1957679.
    Abstract:
    A description is given for the preparation of flow through sensor and of the incorporation of the sensor into a flow injection analysis system. The parameters affecting the measurement are discussed. An accurate, convenient and rapid method is proposed for the determination of berberine-type alkaloids in various Chinese patent medicines (Coptis chinensis Franch., Phellodendron chinesis Schneid., xianglian wan, zuojin wan, ermiao wan and sanmiao wan). The slope is 54-60 mV/decade over the concentration range of 10(-3)-3 x 10(-6) mol/L berberine at pH 2-9.5. Direct potentiometry determination of berberine in various samples showed an average recovery of 99.5-103.5% and a relative standard deviation of 1.3-3.5% at a sampling rate of 120/h.
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