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  • Title: Solvent effect on the UV/Vis absorption and fluorescence spectroscopic properties of berberine.
    Author: Díaz MS, Freile ML, Gutiérrez MI.
    Journal: Photochem Photobiol Sci; 2009 Jul; 8(7):970-4. PubMed ID: 19582272.
    Abstract:
    Spectral and photophysical properties of the alkaloid berberine (B) were studied in solvents with different solvent parameters, using UV/Vis absorption, emission and excitation spectroscopy. The absorption and emission maxima were found to be between 421-431 nm and 514-555 nm, respectively, leading to Stokes' shifts between 4099 and 5735 cm(-1). The fluorescence quantum yields varied between 10(-2)-10(-4), depending on the solvent. Different solvent scales have been used to study the solvatochromism of B. Linear solvation energy relationships (LSER) proposed by Kamlet-Taft suggest that B is a molecule attractive as a probe for solvent polarity and hydrogen bonding properties.
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