These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Prevalence and multiplicity of cutaneous beta papilloma viruses in plucked hairs depend on cellular DNA input.
    Author: Weissenborn SJ, Neale R, de Koning MN, Waterboer T, Abeni D, Bouwes Bavinck JN, Wieland U, Pfister HJ, EPI-HPV-UV-CA Group.
    Journal: J Virol Methods; 2009 Nov; 161(2):280-3. PubMed ID: 19591874.
    Abstract:
    In view of the low loads of beta human papillomaviruses in skin samples, amounts of cellular DNA used in qualitative PCR may become limiting for virus detection and introduce variations in prevalence and multiplicity. This issue was explored within the context of a multicentre study and increasing prevalence and multiplicity was found with increasing input amounts of cellular DNA extracted from hair bulbs. To improve the quality and comparability between different epidemiologic studies ideally equal amounts of cellular DNA should be employed. When cellular DNA input varies this should be clearly taken into account in assessing viral prevalence and multiplicity.
    [Abstract] [Full Text] [Related] [New Search]