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  • Title: Aptamer-Au NPs conjugates-enhanced SPR sensing for the ultrasensitive sandwich immunoassay.
    Author: Wang J, Munir A, Li Z, Zhou HS.
    Journal: Biosens Bioelectron; 2009 Sep 15; 25(1):124-9. PubMed ID: 19592231.
    Abstract:
    The goal of this work is to explore the amplification effect of aptamer-gold nanoparticles (Au NPs) conjugates for ultrasensitive detection of large biomolecules by surface plasmon resonance (SPR). A novel sandwich immunoassay is designed to demonstrate the amplification effect of aptamer-Au NPs conjugates by using human immunoglobulin E (IgE) as model analyte. Human IgE, captured by immobilized goat anti-human IgE on SPR gold film, is sensitively detected by SPR spectroscopy with a lowest detection limit of 1 ng/ml after anti-human IgE aptamer-Au NPs conjugates is used as amplification reagent. Meanwhile, the non-specific adsorption of aptamer-Au NPs conjugates on goat anti-human IgE is confirmed by SPR spectroscopy and then it is minimized by treating aptamer-Au NPs conjugates with 6-mercaptohexan-1-ol (MCH). These results confirm that aptamer-Au NPs conjugates is a powerful sandwich element and an excellent amplification reagent for SPR-based sandwich immunoassay.
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