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  • Title: Thermophilic biohydrogen production by an anaerobic heat treated-hot spring culture.
    Author: Karadag D, Mäkinen AE, Efimova E, Puhakka JA.
    Journal: Bioresour Technol; 2009 Dec; 100(23):5790-5. PubMed ID: 19592235.
    Abstract:
    Batch experiments were conducted to investigate the thermophilic biohydrogen production using an enrichment culture from a Turkish hot spring. Following the enrichment, the culture was heat treated at 100 degrees C for 10 min to select for spore-forming bacteria. H(2) production was accompanied by production of acetate, butyrate, lactate and ethanol. H(2) production was associated by acetate-butyrate type fermentation while accumulation of lactate and ethanol negatively affected the H(2) yield. H(2) production was highest in the temperature range from 49.6 to 54.8 degrees C and optimum values for initial pH and concentrations of iron, yeast extract and glucose were 6.5, 40 mg/l, 4-13.5 g/l, respectively. PCR-DGGE profiling showed that the heat treated culture consisted of species closely affiliated to genus Thermoanaerobacterium.
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