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  • Title: Comparative studies on proteasomes (multicatalytic proteinases) isolated from spermatozoa and eggs of sea urchins.
    Author: Saitoh Y, Kawahara H, Miyamatsu H, Yokosawa H.
    Journal: Comp Biochem Physiol B; 1991; 99(1):71-6. PubMed ID: 1959331.
    Abstract:
    1. Proteasomes (multicatalytic proteinases) have been purified from spermatozoa and eggs of sea urchins by successive chromatographies on DEAE-cellulose, hydroxylapatite, and Sepharose 6B. 2. The isolated sperm and egg proteasomes showed similar properties such as molecular weight (both 630,000) and multiple catalytic activity (pH optimum, substrate specificity, inhibitor-susceptibility and SDS-stimulation). 3. Distinct protein components were detected between sperm and egg proteasomes by SDS-polyacrylamide gel electrophoresis and immunoblot analysis using antibody against egg proteasome.
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