These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Amplified electrochemical aptasensor for thrombin based on bio-barcode method.
    Author: Zhang X, Qi B, Li Y, Zhang S.
    Journal: Biosens Bioelectron; 2009 Sep 15; 25(1):259-62. PubMed ID: 19608403.
    Abstract:
    In the present study, an electrochemical aptasensor for highly sensitive detection of thrombin was developed based on bio-barcode amplification assay. For this proposed aptasensor, capture DNA aptamerI was immobilized on the Au electrode. The functional Au nanoparticles (DNA-AuNPs) are loaded with barcode binding DNA and aptamerII. Through the specific recognition for thrombin, a sandwich format of Au/aptamerI/thrombin/DNA-AuNPs was fabricated. After hybridization with the PbSNPs-labeled barcode DNA, the assembled sensor was obtained. The concentration of thrombin was monitored based on the concentration of lead ions dissolved through differential pulse anodic stripping voltammetric (DPASV). Under optimum conditions, a detection limit of 6.2x10(-15) mol L(-1) (M) thrombin was achieved. In addition, the sensor exhibited excellent selectivity against other proteins.
    [Abstract] [Full Text] [Related] [New Search]