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  • Title: High-performance liquid-chromatographic separation of ofloxacin using a chiral stationary phase.
    Author: Sun X, Wu D, Shao B, Zhang J.
    Journal: Anal Sci; 2009 Jul; 25(7):931-3. PubMed ID: 19609035.
    Abstract:
    A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm x 4.6 mm, 5 microm) column using a mobile-phase system containing hexane-ethanol-methanol-acetic acid-diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the mobile phase has played an important role in enhancing the chromatographic efficiency and resolution between enantiomers.
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