These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Enhanced yield of medium-chain-length polyhydroxyalkanoates from nonanoic acid by co-feeding glucose in carbon-limited, fed-batch culture.
    Author: Sun Z, Ramsay J, Guay M, Ramsay B.
    Journal: J Biotechnol; 2009 Sep 25; 143(4):262-7. PubMed ID: 19632279.
    Abstract:
    Medium-chain-length polyhydroxyalkanoates (MCL-PHAs) were produced in carbon-limited, single-stage, fed-batch fermentations of Pseudomonas putida KT2440 by co-feeding nonanoic acid (NA) and glucose (G) to enhance the yield of PHA from NA. An exponential (mu=0.25 h(-1)) followed by a linear feeding strategy at a NA:G ratio of 1:1 (w/w) achieved 71 g l(-1) biomass containing 56% PHA. Although the same overall PHA productivity (1.44 g l(-1) h(-1)) was obtained when NA alone was fed at the same specific growth rate, the overall yield of PHA from NA increased by 25% (0.66 g PHA g NA(-1) versus 0.53 g g(-1)) with glucose co-feeding. Further increasing glucose in the feed (NA:G=1:1.5) resulted in a slightly higher yield (0.69 g PHA g NA(-1)) but lower PHA content (48%) and productivity (1.16 g l(-1) h(-1)). There was very little change in the PHA composition.
    [Abstract] [Full Text] [Related] [New Search]