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  • Title: Inhibition of P2X receptor-mediated inward current by protein kinase C in small-diameter dorsal root ganglion neurons of adult rats.
    Author: Bie BH, Zhang YH, Zhao ZQ.
    Journal: Neurosci Bull; 2009 Aug; 25(4):179-86. PubMed ID: 19633699.
    Abstract:
    OBJECTIVE: The aim of the present study is to verify the ATP-induced varied responses in isolated dorsal root ganglion (DRG) neurons of the adult rat, and investigate the modulatory effects of specific P2X receptor agonist beta, gamma-me-ATP and protein kinase C (PKC) on P2X receptor-mediated inward current in DRG neurons. METHODS: Whole cell patch-clamp was employed to record the currents on acutely isolated DRG neurons in the adult rats. RESULTS: beta, gamma-me-ATP, similar as ATP, evoked 2 distinct subtypes of P2X receptor-mediated inward currents in a dose-dependent manner in DRG neurons. Activation of PKC by phorbol 12, 13-dibutyrate (PDBu) significantly inhibited both subtypes of inward currents mediated by P2X receptors in a dose-dependent manner. CONCLUSION: Activation of PKC negatively modulated the P2X receptor-mediated currents in rat DRG neurons, which may be of benefit to preventing the over-excitation of nociceptor under inflammatory or neuropathic conditions. 目的: 在成年大鼠背根节神经元, 观察ATP诱导的多样性反应, 研究P2X 激动剂β,γ-me-ATP 以及蛋白激酶 C对P2X受体介导的内向电流的调制作用. 方法: 应用全细胞膜片钳记录急性分离的大鼠背根节神经元电活动. 结果: 与ATP诱导的反应相同, P2X激动剂β,γ-me-ATP也引起P2X受体介导的两种类型的内向电流, 并呈剂量依赖性. 蛋白激酶C 激动剂phorbol 12,13-dibutyrate (PDBu)能显著抑制P2X 受体介导的两类内向电流, 并呈剂量依赖关系. 结论: 蛋白激酶C负向性调制大鼠背根节神经元的P2X 受体介导的内向电流, 提示蛋白激酶C的激动剂可用于防止炎症和神经病理条件下伤害性感受器的过度兴奋.
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