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  • Title: Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay.
    Author: Qu Q, Zhu Z, Wang Y, Zhong Z, Zhao J, Qiao F, Du X, Wang Z, Yang R, Huang L, Yu Y, Zhou L, Chen Z.
    Journal: J Microbiol Methods; 2009 Oct; 79(1):121-3. PubMed ID: 19635504.
    Abstract:
    A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5 x 10(6)CFU/ml and the sensitivity for different spiked samples ranged from 2.0 x 10(3) to 3.9 x 10(5)CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection.
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