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  • Title: Cytochemical demonstration of acid phosphatase, trimetaphosphatase and basic protein in rat peritoneal mast cells during 48/80 induced exocytosis.
    Author: Jamur MC, Vugman I.
    Journal: Cell Mol Biol; 1990; 36(5):509-14. PubMed ID: 1963575.
    Abstract:
    Acid phosphatase (AcP-A), trimetaphosphatase (TmP-A) activities and basic protein reaction were cytochemically studied in rat peritoneal mast cells 15 minutes after stimulation by compound 48/80. The AcPase reaction was positive in slightly altered granules, but negative in those more intensely altered, and also in unaltered granules. The TmP-A reaction was negative in altered granules and positive in a few unaltered granules. These results suggest that mast cells have two populations of granules, one, comprising most granules, is AcP-A positive and is exocytosed. The other, smaller, is TmP-A positive, and is not exocytosed. Intact granules gave a strong positive reaction with amoniacal silver nitrate (AS), which detects basic protein. This reaction decreased in intensity with increasing granule alteration.
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