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  • Title: Detection of Shiga toxin-producing Escherichia coli and enteropathogenic Escherichia coli in faecal samples of healthy mithun (Bos frontalis) by multiplex polymerase chain reaction.
    Author: Rajkhowa S, Das R, Bora S, Rajkhowa C, Rahman H, Bujarbaruah KM.
    Journal: Zoonoses Public Health; 2010 Sep; 57(6):397-401. PubMed ID: 19638162.
    Abstract:
    Faecal samples obtained from 190 healthy mithuns were examined for the presence of Escherichia coli. Total one-hundred and five E. coli isolates were obtained from these samples, which belonged to 55 different serogroups. These isolates were subjected to multiplex polymerase chain reaction (m-PCR) for detection of stx1, stx2, eaeA and hlyA genes. Twenty-three (21.90%) E. coli isolates belonging to 14 serogroups revealed the presence of at least one virulence gene when examined by m-PCR. Nineteen percent and 2.85% of the mithuns were found to carry Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli, respectively. stx1 and stx2 genes were found to be prevalent in 7 (6.67%) and 18 (17.14%) of the isolates respectively, whereas eaeA and hlyA genes were found to be carried by three (2.85% each) isolates. Interestingly, none of the STEC isolates belonged to serogroup O157.
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