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  • Title: Development of reverse-transcription loop-mediated isothermal amplification for the detection of infectious bursal disease virus.
    Author: Xu J, Zhang Z, Yin Y, Cui S, Xu S, Guo Y, Li J, Wang J, Liu X, Han L.
    Journal: J Virol Methods; 2009 Dec; 162(1-2):267-71. PubMed ID: 19643144.
    Abstract:
    To establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of infectious bursal disease virus (IBDV), four primers specific to six regions of the VP3 gene were designed; the VP3 region was selected because it is a conserved part of the IBDV genome. After amplification in an isothermal water bath for 70 min, samples containing IBDV generated the expected ladder-like products while other viruses generated no product. The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with reverse-transcription polymerase chain reaction (RT-PCR) and virus isolation. The assay was significantly more sensitive than normal gel-based RT-PCR. Because it is specific and simple, the RT-LAMP assay can be widely applied in clinical laboratories for rapid detection of IBVD.
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