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  • Title: Chelating ion-exchange methods for the preparation of no-carrier-added 64Cu.
    Author: Watanabe S, Watanabe S, Liang J, Hanaoka H, Endo K, Ishioka NS.
    Journal: Nucl Med Biol; 2009 Aug; 36(6):587-90. PubMed ID: 19647164.
    Abstract:
    INTRODUCTION: We have developed a method for producing no-carrier-added (64)Cu by using chelating resin bearing iminodiacetic acid groups. METHODS: We optimized the conditions for the selective separation of radioactive Cu from Ni and Co using the chelating resin and produced no-carrier-added (64)Cu under the optimized conditions. We analyzed the amounts of the metal ions present in (64)Cu by inductively coupled-plasma mass spectroscopy (ICP-MS) and optical emission spectroscopy (ICP-OES), and performed radiolabeling of monoclonal antibodies in order to investigate the quality of the (64)Cu produced in this study. RESULTS: Radioactive Cu was separated from Ni and Co with 0.1 and 2 M HCl solutions. The yield of (64)Cu isolated from the (64)NiO target was almost 87%. The radiochemical purity of (64)Cu obtained from different amounts of (64)NiO targets was >99% in all cases. We found that the (64)Cu solution presented extremely low amounts of the metal ions and showed high specific activity (average: 595 GBq/mumol). Moreover, the antibodies were labeled with (64)Cu with a high average efficiency (average: 88%). CONCLUSIONS: We could efficiently separate (64)Cu by using short ion-exchange columns. The chelating ion-exchange method provides a high quality of (64)Cu that is sufficient for the synthesis of (64)Cu-labeled antibodies and medical applications.
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