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  • Title: Filter-exchange PGSE NMR determination of cell membrane permeability.
    Author: Aslund I, Nowacka A, Nilsson M, Topgaard D.
    Journal: J Magn Reson; 2009 Oct; 200(2):291-5. PubMed ID: 19647458.
    Abstract:
    A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.
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