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  • Title: [Use of digoxigenin-labeled probes for the detection of papillomaviruses by in situ hybridization].
    Author: Clavel C, Binninger I, Polette M, Boutterin MC, Birembaut P.
    Journal: Ann Pathol; 1990; 10(5-6):351-4. PubMed ID: 1964785.
    Abstract:
    A technique of detection of human papillomaviruses by in situ hybridization in paraffin-embedded formalin-fixed sections of genito-anal lesions is described. The probes are labeled by incorporation of digoxigenin-labeled nucleotides. The hybrids are revealed by immunohistochemistry with an anti-digoxigenin antibody coupled with alkaline phosphatase. This technique is sensitive, cheap and may be used in routine in laboratories of pathology.
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