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  • Title: Purification of rat liver mitochondrial anion carriers by triazine dye affinity chromatography.
    Author: Szewczyk A, Poddana H, Nałecz MJ.
    Journal: Acta Biochim Pol; 1990; 37(1):113-6. PubMed ID: 1965080.
    Abstract:
    Mitochondria were prepared from rat liver, solubilized with Triton X-100 and submitted to chromatography on dry hydroxylapatite. The pass-through from the column, containing a mixture of mitochondrial carriers, was processed further by triazine dye affinity chromatography and optimal conditions for binding and elution of mitochondrial carriers were established. Activities of the mono-, di-, tricarboxylate and 2-oxoglutarate carriers were measured after reconstitution of various chromatographic fractions into asolectin vesicles, and a good resolution of different carriers was obtained. Thus the triazine dye affinity chromatography appears to be a potent new tool for purification of mitochondrial anion carriers.
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